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CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats  期刊论文  

  • 编号:
    03592aeb-6f5b-4999-9c76-1520b28c205b
  • 作者:
    Fu Hongmin#[1]Huangfu Chunrong[2];Zheng Rui[2];Su Lina[2];Wang Yajun[2];Li Li*[2]
  • 语种:
    英文
  • 期刊:
    JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY ISSN:1138-7548 2016 年 73 卷 3 期 (381 - 386) ; AUG
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  • 摘要:

    Calcitonin gene-related peptide (CGRP) has been shown to play important roles in biological functions. However, there is very little evidence on the value of CGRP in lipopolysaccharide (LPS)-induced acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Therefore, this study aimed to investigate the role of CGRP in LPS-induced ALI in rats. In the experiment, Sprague-Dawley (SD) rats were randomized into control, an antagonist of alpha-calcitonin gene-related peptide receptor (CGRP8-37), LPS groups, and CGRP8-37 + LPS groups. ALI model was prepared through retrograde injection of LPS (10 mg/kg). At 6 and 12 h, bron-choalveolar lavage was performed and used to assess total cell count and levels of tumor necrosis factor-alpha, interleukin-1 beta, -6, and -10 by enzyme-linked immunosorbent assay (ELISA). Lung tissue was collected for assessing wet-to-dry (W/D) ratio, hematoxylin and eosin staining. Aquaporin (AQP)-1 and -5 expressions in lung tissues were detected by quantitative PCR and Western blot. The results showed that histological injury, total cell count, and W/D ratio significantly reduced in LPS group after 6 h. The levels of inflammatory cytokines in CGRP8-37 + LPS-treated rats were higher than that in LPS-treated rats (all, P < 0.001). Real-time RT-PCR analysis showed that levels of AQP-1 in rats from CGRP8-37 + LPS group was lower than that in LPS-treated rats (P = 0.005 and P < 0.001). Western blotting analysis showed that AQP-1 protein levels at 6 h significantly decreased in CGRP8-37 + LPS rats. Together, our data suggest that CGRP antagonists, CGRP8-37 could enhance ALI induced by LPS in the rat model, and regulate the expression levels of AQP-1 and AQP-5 by affecting inflammatory cytokines. Thereby, regulating endogenous CGRP may be a potential treatment for ALI/ARDS.

  • 推荐引用方式
    GB/T 7714:
    Fu Hong-min,Huangfu Chun-rong,Zheng Rui, et al. CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats [J].JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY,2016,73(3):381-386.
  • APA:
    Fu Hong-min,Huangfu Chun-rong,Zheng Rui,Su Li-na,&Li Li.(2016).CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats .JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY,73(3):381-386.
  • MLA:
    Fu Hong-min, et al. "CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats" .JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY 73,3(2016):381-386.
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