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Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts 期刊论文

编号：

04252e04-1c60-44d9-abda-64a88063e0c4
作者：

Xiang, Zheng(向征)#^[1,2]Chen, Xinwen#^[3]Yang, Lijun(杨立军)^[2]He, Yongshu(何永蜀)^[2]Jiang, Runsheng(姜润生)^[4]Rosenthal, Benjamin M.^[5];Luan, Pengtao^[1];Attwood, S. W.^[6];Zuo, Yangxian^[3];Zhang, Yaping*^[1,7]Yang, Zhaoqing(杨照青)*^[2]
语种：

英文
期刊：

PARASITOLOGY INTERNATIONAL ISSN：1383-5769 2009 年 58 卷 3 期 (293 - 296) ; SEP
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关键词：

Sarcocystis Oocysts Faeces Taxonomy 18S rRNA Diagnostics
摘要：

Because the excreted sporocysts and/or oocysts of various species of Sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18S rDNA sequence. Oocysts and/or sporocysts; from three taxa of Sarcocystis were collected from human, feline, and canine definitive hosts that had fed upon meats infected with the muscle cysts of Sarcocystis hominis, Sarcocystis fusiformis and a species of Sarcocystis from water buffalo that could not be distinguished from Sarcocystis cruzi. Using a new collection method employing filter paper, these excreted oocysts and sporocysts were subjected to DNA extraction, as were the corresponding muscle cysts. Methods employing PCR-RFLP and DNA sequencing of a partial 18S rDNA gene (ssrRNA) sequence were then used to successfully distinguish among the three taxa. The same, unique restriction digestion pattern characterizes the tissue cysts and oocysts and/or sporocysts of each parasite taxon. The technique makes possible amplification and identification of species specific gene sequences based on DNA extracted from as few as 7 excreted sporocysts (the equivalent of 3 and 1/2 oocysts) from freshly prepared material, or as few as 50 sporocysts from feces samples that had been stored in potassium dichromate (K2Cr2O7) for as long as 6 years. This represents the first report using molecular diagnostic procedures to diagnose oocysts of Sarcocystis in faecal samples, describing a valuable new tool for studying the epidemiology of various Sarcocystis species. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
推荐引用方式
GB/T 7714：

Xiang Zheng,Chen Xinwen,Yang Lijun, et al. Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts [J].PARASITOLOGY INTERNATIONAL,2009,58(3):293-296.
APA：

Xiang Zheng,Chen Xinwen,Yang Lijun,He Yongshu,&Yang Zhaoqing.(2009).Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts .PARASITOLOGY INTERNATIONAL,58(3):293-296.
MLA：

Xiang Zheng, et al. "Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts" .PARASITOLOGY INTERNATIONAL 58,3(2009):293-296.
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