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A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen) 期刊论文

编号：

0c80f676-5aa2-4c6a-ad3a-41fa4e2aa616
作者：

Li, Wenliang(李文亮)#^[1,2]Li, Sisi#^[1]Zhong, Jian#^[3,4]Zhu, Zhu^[2];Liu, Jingze*^[1]Wang, Wenhong*^[3]
语种：

英文
期刊：

PEPTIDES ISSN：0196-9781 2011 年 32 卷 6 期 (1146 - 1150) ; JUN
收录：
关键词：

Earthworm Antimicrobial peptide Skin secretion Lumbricin Pheretima guillelmi
摘要：

A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C(8) reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEMPAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells. (C) 2011 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Li Wenliang,Li Sisi,Zhong Jian, et al. A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen) [J].PEPTIDES,2011,32(6):1146-1150.
APA：

Li Wenliang,Li Sisi,Zhong Jian,Zhu Zhu,&Wang Wenhong.(2011).A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen) .PEPTIDES,32(6):1146-1150.
MLA：

Li Wenliang, et al. "A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen)" .PEPTIDES 32,6(2011):1146-1150.
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