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Ectopic expression of Cripto-1 in transgenic mouse embryos causes hemorrhages, fatal cardiac defects and embryonic lethality 期刊论文

编号：

1303611e-56e5-4166-b3f3-3cb419643f86
作者：

Lin, Xiaolin#^[1,2,3,4,5]Zhao, Wentao#^[3,4,6]Jia, Junshuang#^[3,4]Lin, Taoyan^[1,2,3,4];Xiao, Gaofang^[3,4];Wang, Shengchun^[3,4];Lin, Xia^[3,4];Liu, Yu^[1,2];Chen, Li^[7];Qin, Yujuan^[3,4];Li, Jing^[3,4];Zhang, Tingting^[3,4];Hao, Weichao^[3,4];Chen, Bangzhu^[1,2];Xie, Raoying^[3,4];Cheng, Yushuang^[3,4];Xu, Kang^[8];Yao, Kaitai^[3,4];Huang, Wenhua*^[5]Xiao, Dong*^[1,2,3,4]Sun, Yan*^[9]
语种：

英文
期刊：

SCIENTIFIC REPORTS ISSN：2045-2322 2016 年 6 卷 ; SEP 30
收录：
摘要：

Targeted disruption of Cripto-1 in mice caused embryonic lethality at E7.5, whereas we unexpectedly found that ectopic Cripto-1 expression in mouse embryos also led to embryonic lethality, which prompted us to characterize the causes and mechanisms underlying embryonic death due to ectopic Cripto-1 expression. RCLG/EIIa-Cre embryos displayed complex phenotypes between embryonic day 14.5 (E14.5) and E17.5, including fatal hemorrhages (E14.5-E15.5), embryo resorption (E14.5-E17.5), pale body surface (E14.5-E16.5) and no abnormal appearance (E14.5-E16.5). Macroscopic and histological examination revealed that ectopic expression of Cripto-1 transgene in RCLG/EIIa-Cre embryos resulted in lethal cardiac defects, as evidenced by cardiac malformations, myocardial thinning, failed assembly of striated myofibrils and lack of heartbeat. In addition, Cripto-1 transgene activation beginning after E8.5 also caused the aforementioned lethal cardiac defects in mouse embryos. Furthermore, ectopic Cripto-1 expression in embryonic hearts reduced the expression of cardiac transcription factors, which is at least partially responsible for the aforementioned lethal cardiac defects. Our results suggest that hemorrhages and cardiac abnormalities are two important lethal factors in Cripto-1 transgenic mice. Taken together, these findings are the first to demonstrate that sustained Cripto-1 transgene expression after E11.5 causes fatal hemorrhages and lethal cardiac defects, leading to embryonic death at E14.5-17.5.
推荐引用方式
GB/T 7714：

Lin Xiaolin,Zhao Wentao,Jia Junshuang, et al. Ectopic expression of Cripto-1 in transgenic mouse embryos causes hemorrhages, fatal cardiac defects and embryonic lethality [J].SCIENTIFIC REPORTS,2016,6.
APA：

Lin Xiaolin,Zhao Wentao,Jia Junshuang,Lin Taoyan,&Sun Yan.(2016).Ectopic expression of Cripto-1 in transgenic mouse embryos causes hemorrhages, fatal cardiac defects and embryonic lethality .SCIENTIFIC REPORTS,6.
MLA：

Lin Xiaolin, et al. "Ectopic expression of Cripto-1 in transgenic mouse embryos causes hemorrhages, fatal cardiac defects and embryonic lethality" .SCIENTIFIC REPORTS 6(2016).
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