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BDNF Overexpression Exhibited Bilateral Effect on Neural Behavior in SCT Mice Associated with AKT Signal Pathway 期刊论文

编号：

1b54a2bb-e9f4-4cac-a950-32541f71b72e
作者：

Chen, MeiRong#^[1]Dai, Ping(戴萍)#^[2]Wang, ShuFen(王淑芬)^[2]Song, ShuHua^[3];Wang, HangPing^[3];Zhao, Ya^[2];Wang, TingHua(王廷华)#*^[1,2]Liu, Jia(刘佳)#*^[1]
语种：

英文
期刊：

NEUROCHEMICAL RESEARCH ISSN：0364-3190 2016 年 41 卷 10 期 (2585 - 2597) ; OCT
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关键词：

Spinal cord injury BDNF-overexpression Locomotor function Thermal hyperpathia
摘要：

Spinal cord injury (SCI), a severe health problem in worldwide, was commonly associated with functional disability and reduced quality of life. As the expression of brain-derived neurotrophic factor (BDNF) was substantial event in injured spinal cord, we hypothesized whether BDNF-overexpression could be in favor of the recovery of both sensory function and hindlimb function after SCI. By using BDNF-overexpression transgene mice [CMV-BDNF 26 (CB26) mice] we assessed the role of BDNF on the recovery of neurological behavior in spinal cord transection (SCT) model. BMS score and tail-flick test was performed to evaluate locomotor function and sensory function, respectively. Immunohistochemistry was employed to detect the location and the expression of BDNF, NeuN, 5-HT, GAP-43, GFAP as well as CGRP, and the level of p-AKT and AKT were examined through western blot analysis. BDNF overexpressing resulted in significant locomotor functional recovery from 21 to 28 days after SCT, compared with wild type (WT)+SCT group. Meanwhile, the NeuN, 5-HT and GAP-43 positive cells were markedly increased in ventral horn in BDNF overexpression animals, compared with WT mice with SCT. Moreover, the crucial molecular signal, p-AKT/AKT has been largely up-regulated, which is consistent with the improvement of locomotor function. However, in this study, thermal hyperpathia encountered in sham (CB26) group and WT+SCT mice and further aggravated in CB26 mice after SCT. Also, following SCT, the significant augment of positive-GFAP astrocytes and CGRP fibers were found in WT+SCT mice, and further increase was seen in BDNF over-expression transgene mice. BDNF-overexpression may not only facilitate the recovery of locomotor function via AKT pathway, but also contributed simultaneously to thermal hyperalgesia after SCT.
推荐引用方式
GB/T 7714：

Chen Mei-Rong,Dai Ping,Wang Shu-Fen, et al. BDNF Overexpression Exhibited Bilateral Effect on Neural Behavior in SCT Mice Associated with AKT Signal Pathway [J].NEUROCHEMICAL RESEARCH,2016,41(10):2585-2597.
APA：

Chen Mei-Rong,Dai Ping,Wang Shu-Fen,Song Shu-Hua,&Liu Jia.(2016).BDNF Overexpression Exhibited Bilateral Effect on Neural Behavior in SCT Mice Associated with AKT Signal Pathway .NEUROCHEMICAL RESEARCH,41(10):2585-2597.
MLA：

Chen Mei-Rong, et al. "BDNF Overexpression Exhibited Bilateral Effect on Neural Behavior in SCT Mice Associated with AKT Signal Pathway" .NEUROCHEMICAL RESEARCH 41,10(2016):2585-2597.
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