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Development of real-time PCR assays for the quantitative detection of CD81 receptor gene of hepatitis C virus in Tupaia belangeri 会议论文

编号：

20941e8b-f1d5-4243-b3af-644e23ec2805
作者：

Feng, Yue[0];Feng, Yuemei[1];Bai, Jie[2];Xia, Xueshan[3];Zhao, Wenhua[4];Dai, Jiejie[5];Sun, Xiaomei[6];Li, Qihan[7]
作者单位：

Kunming University of Science and Technology, Faculty of Life Science and Technology,Kunming,China[0];Kunming Medical University, Research Institute of Nutrition and Food Science,Kunming,China[1];Kunming University of Science and Technology, Faculty of Life Science and Technology,Kunming,China[2];Kunming University of Science and Technology, Faculty of Life Science and Technology,Kunming,China[3];Tsinghua University, School of Aerospace,Beijing,China[4];Chinese Academy of Medical Sciences,Beijing,China[5];Chinese Academy of Medical Sciences, 3?Institute of Medical Biology,Beijing,China[6];Chinese Academy of Medical Sciences, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Beijing,China[7];
语种：

英文
会议名称：

Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
收录：
关键词：

CD81 HCV Real-time PCR Taqman probe Tupaia belangeri
摘要：

A real-time PCR assay based on the TaqMan chemistry was developed for reliable and quantitative detection of CD81 in Tupaia belangeri. The assay is performed with the ABI 7300 system using TaqMan probe and primers amplifying 118bp CD81 fragment of Tupaia belangeri. The standard curve for quantitation of target gene showed linearity over an at least 5-log DNA concentration range, represents10³ to10⁷ copies per reaction, with a correlation coefficient of 0.9996 (the slopes value -3.39 VS -3.32). Moreover, this protocol enabled detection of as little as 10 copies of CD81 cDNA in Tupaia belangeri liver tissues . The overall % coefficient of variation (%CV) for this assay was lower 5% with statistical significance due to in intra-assay 1.08% and inter-assay 0.16%, which indicated its high reproducibility. The new assay greatly improves current detection methods for CD81 evaluation, and this is the first report on the standardization and evaluation of a Tupaia belangeri CD81 RNA quantitation assay from China. As TaqMan real-time PCR enable the rapid and easy processing of a large number of samples, and can be used as a tool for monitoring progression of CD81 expression.
推荐引用方式
GB/T 7714：

Feng Yue[0],Feng Yuemei[1],Bai Jie[2], et al. Development of real-time PCR assays for the quantitative detection of CD81 receptor gene of hepatitis C virus in Tupaia belangeri [J].Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009,2009.
APA：

Feng Yue[0],Feng Yuemei[1],Bai Jie[2],Xia Xueshan[3],&Li Qihan[7].(2009).Development of real-time PCR assays for the quantitative detection of CD81 receptor gene of hepatitis C virus in Tupaia belangeri .Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009.
MLA：

Feng Yue[0], et al. "Development of real-time PCR assays for the quantitative detection of CD81 receptor gene of hepatitis C virus in Tupaia belangeri" .Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009(2009).

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