In this paper, the electrocardiogram (ECG) signal detecting, amplifying, anti-aliasing filtering circuits were designed and made to get the analog ECG signal, and then make it necessarily conditioned to meet the requirement of data acquisition card USB-6008( signal frequency: 0.05Hz ~ 159Hz, signal amplification: + 1000 times). Using the data acquisition card USB-6008 developed by NI Company for A/D conversion, digital form of ECG signal may be gotten, and it can be transmitted to a personal computer for further processing by LabVIEW2009 (evaluation version). We carried out multi-resolution analysis of the digital ECG signal and got ECG signal components in different sub bands. For the purpose of wavelet filtering, the ECG signal was reconstructed with the signal components in sub bands which reflect the characteristics of ECG and ideal effect of ECG was obtained. ? 2012 IEEE.