为获得阿昌族G6PDWT和G6PDG487A重组蛋白,研究G6PDG487A的结构和功能改变,从云南省德宏州梁河县杞木寨乡湾中村阿昌族聚集地的G6PD缺陷家系先证者和正常阿昌族个体全血提取RNA,经RT-巢式PCR得cDNA,将cDNA克隆至pMD18-Tsimple载体中并测序;错配碱基经定点突变修复后,目的基因亚克隆至pThioHis(A)载体,构建了阿昌族G6PD基因野生型和G487A突变型原核表达载体:pThioHis(A)-AChang-G6PDWT和pThioHis(A)-AChang-G6PDG487A。用重组质粒转化E.coli Competent Cells DF213(G6...