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Radix puerariae extracts ameliorate paraquat-induced pulmonary fibrosis by attenuating follistatin-like 1 and nuclear factor erythroid 2p45-related factor-2 signalling pathways through downregulation of miRNA-21 expression 期刊论文

编号：

50b97e2f-638a-4387-b315-532b8b9f7699
作者：

Liu, Mingwei#^[1]Liu, Rong(刘荣)#^[1]Wu, Haiying^[1];Li, Yiyun^[1];Su, Meixian^[2];Dong, Minna^[1];Zhang, Wei(张玮)*^[1]Qian, Chuanyun(钱传云)*^[1]
语种：

英文
期刊：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE ISSN：1472-6882 2016 年 16 卷 ; JAN 12
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关键词：

Lung fibrosis Radix puerariae extracts miR-21 Connective tissue growth factor Nuclear factor erythroid 2p45-related factor-2 Nuclear factor-kappa B Oxidative stress Mice
摘要：

Background: Puerarin, extracted from Radix puerariae, was reported to ameliorate airway inflammation, lung injury and lung fibrosis induced by paraquat (PQ) in mice. However, effects of Radix puerariae extracts (RPEs) on lung fibrosis or signalling pathways in PQ-induced lung injury have not been well studied. Therefore, the goals of our study were to investigate whether Radix puerariae extracts are antifibrotic in a paraquat (PQ) induced lung fibrosis model in mice and to propose possible mechanisms of action of the RPE effects.
Methods: We used a long-term exposure model of PQ-induced lung fibrosis in mice to evaluate effects of antioxidant-containing RPE. We examined effects of miR-21 on follistatin-like 1 (Fstl 1) pathways and oxidative stress in the lung. Gene expression levels of miR-21, Fstl 1, transforming growth factor-beta 1 (TGF-beta 1), connective tissue growth factor (CTGF), collagen-1 and collagen III were measured by real-time PCR. Protein expression levels of Fstl 1(FSTL1), heme oxygenase-1 (HO-1), nuclear factor erythroid 2p45-related factor-2 (Nrf2), Smad2/3, p38MAPK, nuclear factor-kappa B 65 (NF-kappa B65), and matrix metalloproteinase-9 were detected by western blotting. FSTL1 andalpha-smooth muscle actin (alpha-SMA) in lung tissue were detected by immunohistochemistry. Malondialdehyde, superoxide dismutase (SOD), reduced (GSH) and oxidised (GSSH) glutathione and reactive oxygen species levels, hydroxyproline and total lung collagen were also determined.
Results: Long-term challenge with PQ enhanced miRNA-21 (miR-21), Fstl 1 pathways, oxidative stress and development of fibrotic features in the lungs. RPE reduced features of lung fibrosis by blocking Fstl 1 pathways and oxidative stress through decreased miR-21 expression. This was accompanied by suppression of CTGF, TGF-beta 1, vascular endothelial growth factor, collagen I, and collagen III. In addition, PQ-induced activation of NF-kappa B, Nrf2 and alpha-SMA were enhanced by puerarin. We also found that puerarin increased HO-1, SOD and GSH levels.
Conclusions: These findings demonstrated that RPEs blocked PQ-induced Fstl 1 pathways and oxidative stress by inhibiting miR-21 expression, leading to attenuation of PQ-induced lung fibrosis.
推荐引用方式
GB/T 7714：

Liu Ming-wei,Liu Rong,Wu Hai-ying, et al. Radix puerariae extracts ameliorate paraquat-induced pulmonary fibrosis by attenuating follistatin-like 1 and nuclear factor erythroid 2p45-related factor-2 signalling pathways through downregulation of miRNA-21 expression [J].BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2016,16.
APA：

Liu Ming-wei,Liu Rong,Wu Hai-ying,Li Yi-yun,&Qian Chuan-yun.(2016).Radix puerariae extracts ameliorate paraquat-induced pulmonary fibrosis by attenuating follistatin-like 1 and nuclear factor erythroid 2p45-related factor-2 signalling pathways through downregulation of miRNA-21 expression .BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,16.
MLA：

Liu Ming-wei, et al. "Radix puerariae extracts ameliorate paraquat-induced pulmonary fibrosis by attenuating follistatin-like 1 and nuclear factor erythroid 2p45-related factor-2 signalling pathways through downregulation of miRNA-21 expression" .BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 16(2016).
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