Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MDSC with LPS plus IFN-gamma inhibited the DC development but enhanced MDSC functions, such as NO release and T cell suppression. In our study, bone marrow precursor cells cultures in GM-CSF and IL-4 were treated with poly(I:C) through the culture, Gr1(+)CD11b(+) cells with MDSC functions, such as NO release and T cell suppression were accumulated in the culture system. Then the similar phenomenon was observed in the vesicular stomatitis virus infection in vivo. In conclusion, we demonstrated that the bone marrow precursor cells in the presence of GM-CSF and IL-4 can differentiate into MDSC, which is dependent on the dynamic of interaction with poly(I:C).
[1]Second Mil Med Univ, Dept Immunol, Shanghai 200433, Peoples R China.
[2]Second Mil Med Univ, Natl Key Lab Med Immunol, Shanghai 200433, Peoples R China.
[3]Second Mil Med Univ, Dept Radiat Med, Shanghai 200433, Peoples R China.
[4]Dept Ctr Dis Prevent & Control, Kunming 650032, Chengdu Mil Reg, Peoples R China.
[5]Sichuan Univ, W China Hosp, Dept Pediat Surg, Chengdu 610064, Peoples R China.
[6]Yuxi People Hosp, Dept Gynaecol & Obstet, Yuxi, Peoples R China.
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