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Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone  期刊论文   WOS高被引论文

  • 编号:
    5518CCC923F496CA9618F246024208AA
  • 作者:
    Zhao, Hui(赵卉)#[1]Liu, Feng#[1]Xie, Wei#[1]Zhou, TaiCheng(周太成)#[2]OuYang, Jun[1];Jin, Lian[1];Li, Hui[2];Zhao, ChunYan[2];Zhang, Liang[2];Wei, Jia(韦嘉)*[2]Zhang, YaPing(张亚平)*[1,3]Li, CanPeng(李灿鹏)*[4]
  • 语种:
    英文
  • 期刊:
    SENSORS AND ACTUATORS B-CHEMICAL ISSN:0925-4005 2021 年 327 卷 ; JAN 15
  • 收录:
  • 关键词:
  • 摘要:

    The recent pandemic outbreak of COVID-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to public health globally. Thus, developing a rapid, accurate, and easy-to-implement diagnostic system for SARS-CoV-2 is crucial for controlling infection sources and monitoring illness progression. Here, we reported an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting RNA of SARS-CoV-2. Based on a supersandwich-type recognition strategy, the technology was confirmed to practicably detect the RNA of SARS-CoV-2 without nucleic acid amplification and reverse-transcription by using a portable electrochemical smartphone. The biosensor showed high specificity and selectivity during in silico analysis and actual testing. A total of 88 RNA extracts from 25 SARS-CoV-2-confirmed patients and eight recovery patients were detected using the biosensor. The detectable ratios (85.5 % and 46.2 %) were higher than those obtained using RT-qPCR (56.5 % and 7.7 %). The limit of detection (LOD) of the clinical specimen was 200 copies/mL, which is the lowest LOD among the published RNA measurement of SARS-CoV-2 to date. Additionally, only two copies (10 mu L) of SARS-CoV-2 were required for per assay. Therefore, we developed an ultrasensitive, accurate, and convenient assay for SARS-CoV-2 detection, providing a potential method for point-of-care testing.

  • 推荐引用方式
    GB/T 7714:
    Zhao Hui,Liu Feng,Xie Wei, et al. Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone [J].SENSORS AND ACTUATORS B-CHEMICAL,2021,327.
  • APA:
    Zhao Hui,Liu Feng,Xie Wei,Zhou Tai-Cheng,&Li Can-Peng.(2021).Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone .SENSORS AND ACTUATORS B-CHEMICAL,327.
  • MLA:
    Zhao Hui, et al. "Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone" .SENSORS AND ACTUATORS B-CHEMICAL 327(2021).
  • 入库时间:
    2021/3/24 5:11:43
  • 更新时间:
    2021/3/24 5:11:43
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