Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin. 4',6-Diamidino-2-phenylindole labelled BM-MSCs were injected into tree shrews. The DN tree shrew model was successfully established. Blood glucose was significantly increased (p < 0.01) during the entire experiment. DN tree shrews showed dyslipidemia, insulin resistance and increased 24-h proteinuria. At 21 days after BM-MSC transplantation, glucose and levels of triglycerides, total cholesterol and 24-h urine volume were lower than in tree shrews with DN alone (p < 0.01) but were still higher than control values (p < 0.01). Levels of creatinine and urea nitrogen as well as 24-h proteinuria were lower for DN tree shrews with BM-MSCs transplantation than DN alone (p < 0.05). High-sugar and high-fat diet combined with STZ injection can induce a tree shrew model of DN. BM-MSCs injection can home to damaged kidneys and pancreas, for reduced 24-h proteinuria and improved insulin resistance. Copyright (C) 2014 John Wiley & Sons, Ltd.
[1]Chengdu Mil Command, Kunming Gen Hosp, Stem Cell Engn Lab Yunnan Prov, Kunming 650032, Peoples R China.
[2]Kunming Med Univ, Chengdu Mil Command, Kunming Gen Hosp, Clin Coll, Kunming, Peoples R China.
[3]Chinese Acad Med Sci, Inst Med Biol, Ctr Tree Shrew Germplasm Resources, Yunnan Key Lab Vaccine Res & Dev Severe Infect Di, Kunming 650118, Peoples R China.
[4]Peking Union Med Coll, Kunming 650118, Peoples R China.
(8.0+极速模式)