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Viral IL-10 down-regulates the "MHC-I antigen processing operon" through the NF-kappa B signaling pathway in nasopharyngeal carcinoma cells 期刊论文

编号：

73b2b7ab-a2b1-42e3-ae32-ab03d739342e
作者：

Ren, Yanxin(任艳鑫)#^[1]Yang, Jie(杨洁)#^[1]Sun, Ruimei(孙瑞梅)^[1]Zhang, LiJuan(张丽娟)^[3]Zhao, LiuFang^[1];Li, BaoZhong^[1];Li, Lei^[1];Long, HaiTing^[4];Sun, QiangMing^[4];Huang, YunChao(黄云超)^[2]Li, Xiaojiang(李晓江)*^[1]
语种：

英文
期刊：

CYTOTECHNOLOGY ISSN：0920-9069 2016 年 68 卷 6 期 (2625 - 2636) ; DEC
收录：
关键词：

Nasopharyngeal neoplasms Nuclear factor Kappa B (NF-kappa B) Viral interleukin-10 (vIL-10) Antigen presentation
摘要：

The HLA-I antigen processing machinery (APM) plays a crucial role in the anticancer immune response. The loss of surface expression of HLA-I molecules is particularly important as this enables tumor cells to evade recognition and lysis by cytotoxic T-lymphocytes. Transcriptional control of the APM genes is regulated by the nuclear factor kappa B (NF-kappa B). BCRFl is an Epstein-Barr virus homologue of human IL-10 (hIL-10) and is known as viral IL-10 (vIL-10). vIL-10 shares many immunosuppressive effects with hIL-10 but lacks the immunostimulatory effect of hIL-10. The aim of this study was to assess whether vIL-10 inhibits APM components (TAP-1, TAP-2, LMP-2, LMP-7 and HLA-I) through the NF-kappa B signaling pathway in nasopharyngeal carcinoma. This work demonstrated that vIL-10 inhibited NF-kappa B activation by blocking IKK phosphorylation and promoting the expression of IKB. TNF-alpha treatment led to a strong translocation of NF-kappa B p65, whereas pretreatment with vIL-10 before TNF-alpha treatment blocked NF-kappa B p65 translocation. vIL-10 also inhibited TNF-alpha-induced DNA-binding of NF-kappa B p65 in the nucleus. Furthermore, chromatin immunoprecipitation analysis demonstrated that NF-kappa B p65 could bind to the TAP-1, TAP-2, LMP-2, LMP-7 and HLA-I gene promoters, and after TNF-alpha stimulation, the down-regulation of TAP-1, TAP-2, LMP-2, LMP-7 and HLA-I transcription by vIL-10 correlated with the suppression of NF-kappa B in CNE-2 cells. Surprisingly, vIL-10 inhibits only TAP-1 and LMP-7 transcription in CNE-1 cells. Taken together, these results suggest that the inhibition of NF-kappa B activity may be an important mechanism for vIL-10 suppression of APM (TAP-1, TAP-2, LMP-2, LMP-7 and HLA-I) gene transcription in CNE-2 cells.
推荐引用方式
GB/T 7714：

Ren Yan-xin,Yang Jie,Sun Rui-mei, et al. Viral IL-10 down-regulates the "MHC-I antigen processing operon" through the NF-kappa B signaling pathway in nasopharyngeal carcinoma cells [J].CYTOTECHNOLOGY,2016,68(6):2625-2636.
APA：

Ren Yan-xin,Yang Jie,Sun Rui-mei,Zhang Li-Juan,&Li Xiao-jiang.(2016).Viral IL-10 down-regulates the "MHC-I antigen processing operon" through the NF-kappa B signaling pathway in nasopharyngeal carcinoma cells .CYTOTECHNOLOGY,68(6):2625-2636.
MLA：

Ren Yan-xin, et al. "Viral IL-10 down-regulates the "MHC-I antigen processing operon" through the NF-kappa B signaling pathway in nasopharyngeal carcinoma cells" .CYTOTECHNOLOGY 68,6(2016):2625-2636.
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