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Diagnostic value of miR-133 in aortic dissection and its mechanism 期刊论文

编号：

7b0f15d6-0982-4377-976b-e347de0dca20
作者：

Ma, Runwei(马润伟)#^[1]Wang, Jiyang^[1];Wu, Xingjian^[2];Chen, Zongning^[3];Zhang, Xiaoyu^[1];Yang, Xu^[1];Song, Yi*^[1]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY ISSN：1936-2625 2017 年 10 卷 3 期 (2804 - 2813)
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关键词：

Acute myocardial infarction aortic dissection miRNAs differential diagnosis SOX4
摘要：

Background: Acute aortic dissection (AD) is a life-threatening condition that remains challenging to diagnose and treat. MiR-133 plays important roles in aortic disease initiation and progression. However, its differential diagnostic value between acute myocardial infarction (AMI) and AD remains unclear. Methods: Serum samples were collected from healthy individuals, AMI and AD patients. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess miR-133 expression. The differential diagnostic value values of miR-133 were analysed by statistical methods. Tissue samples were collected from AMI and AD patients, RT-PCR was used to test the expression of miR-133 and SOX4 mRNA, and Western blot and gelatin zymography were used to test the expression and activity of MMP-2, TIMP-1 and TIMP-2. In vitro, miR-133 mimics was transfected into VSMCs, and the biological function of miR-133 was evaluated by MTT assay, transwell assays, Western blot and gelatin zymography. A luciferase assay was used to explore whether SOX4-3'-UTR was a target gene of miR-133. Results: Compared with AMI patients, AD patients showed reduced serum miR-133 levels. In addition, ROC curve analysis shown that the sensitivity and specificity of serum miR-133 were 90.4% and 58.70% respectively for differential diagnoses between AD and AMI. In vitro, miR-133 up-regulation resulted in reduced VSMCs proliferation and invasion. In addition, an inverse correlation between miR-133 levels and SOX4 mRNA amounts was observed in AD tissues. More importantly, miR133 up-regulation resulted in reduced SOX4 expression, and the luciferase activity of the SOX4-3'-UTR plasmid was significantly suppressed by miR-133. Conclusion: MiR-133 has good application in differential diagnosis between AMI and AD. MiR-133 targets SOX4, thereby inhibiting VSMCs proliferation and migration and finally prevents the development of AD.
推荐引用方式
GB/T 7714：

Ma Runwei,Wang Jiyang,Wu Xingjian, et al. Diagnostic value of miR-133 in aortic dissection and its mechanism [J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2017,10(3):2804-2813.
APA：

Ma Runwei,Wang Jiyang,Wu Xingjian,Chen Zongning,&Song Yi.(2017).Diagnostic value of miR-133 in aortic dissection and its mechanism .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,10(3):2804-2813.
MLA：

Ma Runwei, et al. "Diagnostic value of miR-133 in aortic dissection and its mechanism" .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10,3(2017):2804-2813.
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