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Immunization of stromal cell targeting fibroblast activation protein providing immunotherapy to breast cancer mouse model  期刊论文  

  • 编号:
    7e167a37-170e-4f0f-ad89-9c72aad1754b
  • 作者:
    Meng, Mingyao(孟明耀)#[1,2]Wang, Wenju#[1,2]Yan, Jun[1];Tan, Jing[1];Liao, Liwei[1];Shi, Jianlin[1,2];Wei, Chuanyu[1,2];Xie, Yanhua[1,2];Jin, Xingfang[1,2];Yang, Li[1];Jin, Qing[1];Zhu, Huirong[1];Tan, Weiwei[1,2];Yang, Fang(杨芳)*[1,2,3]Hou, Zongliu(侯宗柳)*[1,2]
  • 语种:
    英文
  • 期刊:
    TUMOR BIOLOGY ISSN:1010-4283 2016 年 37 卷 8 期 (10317 - 10327) ; AUG
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  • 关键词:
  • 摘要:

    Unlike heterogeneous tumor cells, cancer-associated fibroblasts (CAF) are genetically more stable which serve as a reliable target for tumor immunotherapy. Fibroblast activation protein (FAP) which is restrictively expressed in tumor cells and CAF in vivo and plays a prominent role in tumor initiation, progression, and metastasis can function as a tumor rejection antigen. In the current study, we have constructed artificial FAP(+) stromal cells which mimicked the FAP(+) CAF in vivo. We immunized a breast cancer mouse model with FAP(+) stromal cells to perform immunotherapy against FAP(+) cells in the tumor microenvironment. By forced expression of FAP, we have obtained FAP(+) stromal cells whose phenotype was CD11b(+)/CD34(+)/Sca-1(+)/FSP-1(+)/MHC class I+. Interestingly, proliferation capacity of the fibroblasts was significantly enhanced by FAP. In the breast cancer-bearing mouse model, vaccination with FAP(+) stromal cells has significantly inhibited the growth of allograft tumor and reduced lung metastasis indeed. Depletion of T cell assays has suggested that both CD4(+) and CD8(+) T cells were involved in the tumor cytotoxic immune response. Furthermore, tumor tissue from FAP-immunized mice revealed that targeting FAP(+) CAF has induced apoptosis and decreased collagen type I and CD31 expression in the tumor microenvironment. These results implicated that immunization with FAP(+) stromal cells led to the disruption of the tumor microenvironment. Our study may provide a novel strategy for immunotherapy of a broad range of cancer.

  • 推荐引用方式
    GB/T 7714:
    Meng Mingyao,Wang Wenju,Yan Jun, et al. Immunization of stromal cell targeting fibroblast activation protein providing immunotherapy to breast cancer mouse model [J].TUMOR BIOLOGY,2016,37(8):10317-10327.
  • APA:
    Meng Mingyao,Wang Wenju,Yan Jun,Tan Jing,&Hou Zongliu.(2016).Immunization of stromal cell targeting fibroblast activation protein providing immunotherapy to breast cancer mouse model .TUMOR BIOLOGY,37(8):10317-10327.
  • MLA:
    Meng Mingyao, et al. "Immunization of stromal cell targeting fibroblast activation protein providing immunotherapy to breast cancer mouse model" .TUMOR BIOLOGY 37,8(2016):10317-10327.
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