Stroke is one of the most common diseases that caused high mortality and has become burden to the health care systems. Stem cell transplantation has shown therapeutic effect in ameliorating ischemic damage after cerebral artery occlusion mainly due to their neurogenesis, immune regulation, or effects on the plasticity, proliferation, and survival of host cells. Recent studies demonstrated that skin-derived precursor cells (SKPs) could promote central nervous system regeneration in spinal cord injury model or the neonatal peripheral neuron. Here, we investigated the therapeutic potential of SKPs in a rat model of cerebral ischemia. SKPs were isolated, expanded, and transplanted into rat cortex and striatum after transient middle cerebral artery occlusion. Our results revealed that SKPs transplantation could improve the behavioral measures of neurological deficit. Moreover, immunohistology confirmed that SKPs could secrete basic FGF and VEGF in the ischemic region and further markedly increase the proliferation of endogenous nestin(+) and beta III-tubulin(+) neural stem cells. Furthermore, increased angiogenesis induced by SKPs was observed by vWF and alpha-SMA staining. These data suggest that SKPs induced endogenous neurogenesis and angiogenesis and protected neuron from hypoxic-ischemic environment. In conclusion, SKPs transplantation may be a promising approach in treatment of stroke.
[1]Nankai Univ, State Key Lab Med Chem Biol, Key Lab Bioact Mat, Minist Educ,Coll Life Sci, Tianjin 300071, Peoples R China.
[2]Tianjin Med Univ, Tianjin Inst Urol, Hosp 2, Dept Urol, Tianjin 300211, Peoples R China.
[3]Kunming Med Univ, Affiliated Hosp 2, Dept Endocrinol, Kunming 650101, Yunnan, Peoples R China.
[4]Nankai Univ, Sch Med, Dept Anat, Tianjin 300071, Peoples R China.
(8.0+极速模式)