Septin is a conserved eukaryotic family of GTP-binding filament-forming proteins with functions in cytokinesis and other processes. It has been suggested that the dynamic assembly of septin, including the processes from septin initially localizing to the presumptive bud site to the septin collar finally splitting into two cells, coordinates closely with the checkpoint response of cell cycle. Here, we discovered that over-expression of Alcohol sensitive Ring/PHD finger 1 protein (Asr1p) in Saccharomyces cerevisiae triggered the Swe1p-dependent cell cycle checkpoint for a G2/M transition delay, and this G2/M transition delay was caused by the septin defect. Since it was shown that Asr1p affected actin dynamics through the interaction with Crnlp and crn1 should be epistatic to asr1 in the regulation of actin, the gene knockout of crn1 in the Asr1 p over-expression strain restored the defects in septin and cell cycle along with the disordered actin dynamics. Our investigation further showed that the disturbed septin assembly caused by abnormal Asr1p lead to the abnormal localization of the checkpoint proteins such as Gla4/PAK and Cdc5/Polo, and finally triggered the Swe1p-dependent G2/M transition arrest. Additionally, the Ring finger/PHD domains of Asr1p were illustrated to be required but not sufficient for its role in septin. Taken together, our current data suggested a close relationship in the assembly between septin and actin cytoskeleton, which also partially explained how actin cytoskeleton participated in the regulation of the checkpoint of G2/M. (C) 2015 Elsevier Inc. All rights reserved.
[1]Yunnan Univ, Lab Conservat & Utilizat Bioresources, Minist Educ, Kunming, Peoples R China.
[2]Yunnan Univ, Key Lab Microbial Resources, Minist Educ, Kunming, Peoples R China.
[3]Kunmin Med Coll, Affiliated Hosp 2, Dept Neurosurg, Kunming 650101, Peoples R China.
(8.0+极速模式)