In this study, recombinant baculovirus carrying the microdystrophin and beta-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and beta-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double khock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/beta-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and beta-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/beta-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.
[1]Sun Yat Sen Univ, Dept Neurol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China.
[2]Guangdong Acad Med Sci, Dept Pediat, Guangdong Gen Hosp, Guangdong Neurosci Inst, Guangzhou 510080, Guangdong, Peoples R China.
[3]Affiliated Hosp 1, Dept Neurol, Kunming Med Coll, Kunming 650032, Yunnan Province, Peoples R China.
[4]So Med Univ, Dept Neurol, Nanfang Hosp, Guangzhou 510515, Guangdong, Peoples R China.
[5]Sun Yat Sen Univ, Ctr Stem Cell Biol & Tissue Engn, Guangzhou 510085, Guangdong, Peoples R China.
(8.0+极速模式)