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Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli 期刊论文

编号：

b107028b-e309-44d6-9a8b-2940029d1967
作者：

Qiang, Yan Zhi[0]^[1] Qin, Tong[1]^[1] Fu, Wang[2]^[2] Cheng, WuPei[3]^[3] Li, Yang Sheng[4]^[1] Yi, Gong[5]^[4]
地址：

[1]Chinese Academy of Sciences, SIBS,Beijing,China
[2]The First Affiliated Hospital of Kunming Medical University, Department of Laboratory Medicine,Kunming,China
[3]Huashan Hospital, Institute of Antibiotics,Shanghai,China
[4]University of Chinese Academy of Sciences, Research Center of Biotechnology,Beijing,China
语种：

英文
期刊：

Journal of Antimicrobial Chemotherapy ISSN：0305-7453 2002 年 49 卷 3 期 (549 - 552)
收录：
摘要：

Four amino acid substitutions, two in GyrA and two in ParC subunits of DNA gyrase and topoisomerase IV, respectively, are commonly responsible for fluoroquinolone resistance in Escherichia coli. In this study, an economical and time-efficient mismatch amplification mutation assay (MAMA) PCR was developed to detect mutations in the chromosomal gyrA and parC genes causing these substitutions. One hundred and twenty-one clinical E. coli isolates were tested by this assay, and the results confirmed that accumulation of amino acid alterations in GyrA and ParC correlates closely with stepwise increases in the MIC of ciprofloxacin.
推荐引用方式
GB/T 7714：

Qiang Yan Zhi/7004610349[0],Qin Tong/7006292635[1],Fu Wang/7202947247[2], et al. Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli [J].Journal of Antimicrobial Chemotherapy,2002,49(3):549-552.
APA：

Qiang Yan Zhi/7004610349[0],Qin Tong/7006292635[1],Fu Wang/7202947247[2],Cheng WuPei/36990984400[3],&Yi Gong/7101660713[5].(2002).Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli .Journal of Antimicrobial Chemotherapy,49(3):549-552.
MLA：

Qiang Yan Zhi/7004610349[0], et al. "Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli" .Journal of Antimicrobial Chemotherapy 49,3(2002):549-552.

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