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Expressional difference, distributions of TGF-beta 1 in TGF-beta 1 knock down transgenic mouse, and its possible roles in injured spinal cord 期刊论文

编号：

c91ffc29-c512-4bf3-800e-78f28235fd80
作者：

XiYang, YanBin(习杨彦彬)#^[1]Lu, BingTuan#^[1]YaZhao^[1];YuanZhang^[1];Xia, QinJie^[2];Zou, Yu^[2];Zhang, Wei^[3];Quan, XiongZhi^[3];Liu, Su(刘苏)^[1,3,4,5]McDonald, John W.^[3,4,5];Zhang, LianFeng#*^[6,7]Wang, TingHua(王廷华)*^[1,2]
语种：

英文
期刊：

EXPERIMENTAL BIOLOGY AND MEDICINE ISSN：1535-3702 2014 年 239 卷 3 期 (320 - 329) ; MAR
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关键词：

TGF-beta 1 knockdown transgenic mouse protein expression distribution and localization phenotype
摘要：

Transforming growth factor beta 1 (TGF-beta 1) is a multi-functional cytokine implicated in many aspects of mammalian wound healing and scar tissue formation. However, few experiments have so far addressed the potential biological effects of TGF-beta 1 in the nervous system after injury, in addition to the immune system. In the present study, expressional silencing TGF-beta 1 was achieved by selecting predesigning hairpins targeting mouse TGF-beta 1 genes. Four homozygous transgenic offspring were generated and designed as Founder 90, Founder 12, Founder 41 and Founder 46. The down-regulated rates of TGF-beta 1 in different transgenic mice were also determined. To investigate the potential roles of TGF-beta 1, we observed changes in the neurological behavior of TGF-beta 1-knockdown (TGF-beta 1-kd) mice after spinal cord transection (SCT). Moreover, mRNA levels of inflammatory cytokines, including IL-1, IL-6, IL-10, NF-kappa B and TNF, were also detected in nucleate cells from blood by real-time PCR. Consequently, different TGF-beta 1 expressions were detected in multiple tissues, and protein levels of TGF-beta 1 decreased at different rates relative to that of wild type (WT) ones. The levels of TGF-beta 1 proteins in TGF-beta 1-kd mice decreased at most by 57% in Founder 90, which showed a significant recovery in Basso, Beattie, Bresnahan (BBB) scores after SCT compared with that of WT. However, expressions of immune relative genes showed no dramatic difference compared with WT ones. This study is the first to generate TGF-beta 1 down regulated mice and determine the possible roles of TGF-beta 1 in vivo in different conditions.
推荐引用方式
GB/T 7714：

XiYang Yan-Bin,Lu Bing-Tuan,Ya-Zhao, et al. Expressional difference, distributions of TGF-beta 1 in TGF-beta 1 knock down transgenic mouse, and its possible roles in injured spinal cord [J].EXPERIMENTAL BIOLOGY AND MEDICINE,2014,239(3):320-329.
APA：

XiYang Yan-Bin,Lu Bing-Tuan,Ya-Zhao,Yuan-Zhang,&Wang Ting-Hua.(2014).Expressional difference, distributions of TGF-beta 1 in TGF-beta 1 knock down transgenic mouse, and its possible roles in injured spinal cord .EXPERIMENTAL BIOLOGY AND MEDICINE,239(3):320-329.
MLA：

XiYang Yan-Bin, et al. "Expressional difference, distributions of TGF-beta 1 in TGF-beta 1 knock down transgenic mouse, and its possible roles in injured spinal cord" .EXPERIMENTAL BIOLOGY AND MEDICINE 239,3(2014):320-329.
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