首页 / 院系成果 / 成果详情页

Stromal cell-derived factor-1伪 and transforming growth factor-尾1 synergistically facilitate migration and chondrogenesis of synovium-derived stem cells through MAPK pathways  期刊论文  

  • 编号:
    d5315539-cc39-4bd2-807f-b4d02cfeebb8
  • 作者:
    Wang, Yiming[0][1] Chen, Jifei[1][2] Fan, Wenshuai[2][1] Zhang, Jing[3][3] Hua, Bingxuan[4][1] Sun, Bolin[5][1] Zhu, Liang[6][4] Niu, Xinhao[7][1] Yan, Zuoqin[8][1] Guo, Changan[9][2]
  • 地址:

    [1]Fudan University, Department of Orthopaedics,Shanghai,China

    [2]Fudan University, Department of Orthopaedic Surgery,Shanghai,China

    [3]The Second Affiliated Hospital of Kunming Medical University, Department of Anesthesiology,Kunming,China

    [4]Fudan University,Shanghai,China

  • 语种:
    英文
  • 期刊:
    American Journal of Translational Research ISSN:1943-8141 2017 年 9 卷 5 期 (2656 - 2667)
  • 收录:
  • 关键词:
  • 摘要:

    The clinical translation of tissue engineering methods is confined by the limited external cell sources, which is hopefully to be addressed by the cell guidance approach as cytokine-induced homing and differentiation of the patients’ autologous cells. Synovium-derived stem cells (SDSCs) are a potent cell source for cartilage restoration due to its intrinsic proximity and tissue-specific chondrogenic capacity. In this study, stromal cell-derived factor-1α (SDF-1α) in combination with transforming growth factor β1 (TGF-β1) were used to induce SDSCs migration and chondrogenesis in vitro. The migration capacity was evaluated by transwell assay and for chondrogenic evaluation, the expression of Sox9, ACAN and COL2A1 were assessed by quantitative RT-PCR while the expression of sulfated GAG and collagen II were evaluated by Alcian Blue stain and immunohistochemistry respectively. Our data showed that SDF-1α/CXC chemokine receptor 4 (CXCR4) was involved in SDSCs migration through phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Exogenous TGF-β1 enhanced SDF-1α-induced SDSCs migration in a concentration and time-dependent manner through CXCR4, evidenced as complete blockage by AMD3100, the CXCR4 antagonist and this effect was mediated by extracellular regulated protein kinases (ERK) activation. Moreover, the addition of SDF-1α augmented the TGF-β1-induced SDSCs chondrogenesis, evidenced by the increased pellet sizes and the expressions of COL 2A1, ACAN and Sox9. This effect was related to c-Jun N-terminal kinase (JNK) activation. Collectively, these results suggest that SDF-1α and TGF-β1 interacts with each other and synergistically enhance the SDSCs migration and chondrogenesis through MAPK pathways. © 2017, E-Century Publishing Corporation. All rights reserved.

  • 推荐引用方式
    GB/T 7714:
    Wang Yiming/56728956100[0],Chen Jifei/37001467600[1],Fan Wenshuai/56395927500[2], et al. Stromal cell-derived factor-1伪 and transforming growth factor-尾1 synergistically facilitate migration and chondrogenesis of synovium-derived stem cells through MAPK pathways [J].American Journal of Translational Research,2017,9(5):2656-2667.
  • APA:
    Wang Yiming/56728956100[0],Chen Jifei/37001467600[1],Fan Wenshuai/56395927500[2],Zhang Jing/56823647000[3],&Guo Changan/8127630100[9].(2017).Stromal cell-derived factor-1伪 and transforming growth factor-尾1 synergistically facilitate migration and chondrogenesis of synovium-derived stem cells through MAPK pathways .American Journal of Translational Research,9(5):2656-2667.
  • MLA:
    Wang Yiming/56728956100[0], et al. "Stromal cell-derived factor-1伪 and transforming growth factor-尾1 synergistically facilitate migration and chondrogenesis of synovium-derived stem cells through MAPK pathways" .American Journal of Translational Research 9,5(2017):2656-2667.
浏览次数:141 下载次数:0
浏览次数:141
下载次数:0
打印次数:0
浏览器支持: Google Chrome   火狐   360浏览器极速模式(8.0+极速模式) 
返回顶部