C-19-diterpenoid alkaloids are the main components of Aconitum duclouxii Levl. The process of separation and purification of these compounds in previous studies was tedious and time consuming, requiring multiple chromatographic steps, thus resulted in low recovery and high cost. In the present work, five C-19-diterpenoid alkaloids, namely, benzoylaconine (1), N-deethylaconitine (2), aconitine (-), deoxyaconitine (4), and ducloudine A (5), were efficiently prepared from A. duclouxii Levl (Aconitum L.) by ethyl acetate extraction followed with counter-current chromatography. In the process of separation, the critical conditions of counter-current chromatography were optimized. The two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water/NH3 center dot H2O (25%) (1: 1: 1: 1: 0.1, v/v) was selected and 148.2 mg of 1, 24.1 mg of 2, 250.6 mg of -, 7-.9 mg of 4, and 31.4 mg of 5 were obtained from 1 g total Aconitum alkaloids extract, respectively, in a single run within 4 h. Their purities were found to be 98.4, 97.2, 98.2, 96.8, and 96.6%, respectively, by ultra-high performance liquid chromatography analysis. The presented separation and purification method was simple, fast, and efficient, and the obtained highly pure alkaloids are suitable for biochemical and toxicological investigation.
[1]Zunyi Med Univ, Dept Pharmaceut Sci, Zhuhai 519041, Guangdong, Peoples R China.
[2]Kunming Med Univ Chenggong New City, Sch Pharmaceut Sci, Kunming, Yunnan, Peoples R China.
[3]Kunming Med Univ Chenggong New City, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunnan, Peoples R China.
[4]Zunyi Med Univ, Dept Pharmaceut Anal, Zhuhai 519041, Guangdong, Peoples R China.
[5]Zunyi Med Univ, Dept English, Zhuhai 519041, Guangdong, Peoples R China.
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