首页 / 院系成果 / 成果详情页

NEDD4 enhances bone-tendon healing in rotator cuff tears by reducing fatty infiltration 期刊论文

编号：

159C593BAC5978DC7737E398CE82F10A
作者：

Li, Jian#^[1,2]Peng, Ying#^[3]Zhen, Dong^[2];Guo, Caifen*^[1,4]Peng, Wuxun*^[1,5]
语种：

英文
期刊：

MOLECULAR MEDICINE REPORTS ISSN：1791-2997 2025 年 31 卷 3 期 ; MAR
收录：
关键词：

NEDD4 rotator cuff tear mTOR signaling pathway fatty infiltration skeletal muscle
摘要：

Rotator cuff tears (RCT) can cause shoulder pain, weakness and stiffness, significantly affecting daily life. Analysis of the GSE103266 dataset revealed significant changes in the mTOR/PI3K/Akt signaling pathway and lipid metabolism-related pathways, suggesting that fatty infiltration may affect RCT. The analysis indicated that the ubiquitin ligase NEDD4 plays a critical role in RCT. NEDD4 was found to be highly associated with the mTOR/PI3K/Akt signaling pathway. An RCT model in Sprague-Dawley (SD) rats was established to study the role of NEDD4 in regulating the mTOR pathway and investigate its effects on fatty infiltration. SD rats were divided into NEDD4 overexpression and knockout groups. Tissue recovery, apoptosis and fat deposition were measured through histological staining, reverse transcription-quantitative PCR and western blotting. Additionally, cell culture of fibro-adipogenic progenitors and lentiviral transfection were conducted to investigate the effect of NEDD4 on adipocyte differentiation. NEDD4 overexpression significantly reduced lipid accumulation, whereas NEDD4 knockdown enhanced lipid accumulation. NEDD4 was found to regulate the mTOR pathway and the expression of adipogenesis-related genes, promoting fat metabolism and inhibiting adipocyte differentiation. Histological analysis indicated that NEDD4 overexpression improved tissue recovery and reduced apoptosis. Targeting NEDD4 offers a potential therapeutic strategy to improve the clinical outcomes of patients with RCT by modulating the mTOR pathway and fat metabolism.
推荐引用方式
GB/T 7714：

Li Jian,Peng Ying,Zhen Dong, et al. NEDD4 enhances bone-tendon healing in rotator cuff tears by reducing fatty infiltration [J].MOLECULAR MEDICINE REPORTS,2025,31(3).
APA：

Li Jian,Peng Ying,Zhen Dong,Guo Caifen,&Peng Wuxun.(2025).NEDD4 enhances bone-tendon healing in rotator cuff tears by reducing fatty infiltration .MOLECULAR MEDICINE REPORTS,31(3).
MLA：

Li Jian, et al. "NEDD4 enhances bone-tendon healing in rotator cuff tears by reducing fatty infiltration" .MOLECULAR MEDICINE REPORTS 31,3(2025).
入库时间：

2025/1/7 12:07:57
更新时间：

2025/2/11 21:23:47
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：15  下载次数：0

分享到：

浏览次数：15

下载次数：0

打印次数：0