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The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer 期刊论文

编号：

3919dfe0-4532-40dd-a20d-ed6627b80b2e
作者：

Yao, Hong#^[1,2]Ng, Samuel S.^[1];Tucker, Wesley O.^[1];Tsang, YukKaiTiu^[1];Man, Kwan^[3]Wang, Xiaomei(王晓梅)^[4]Chow, Billy K. C.^[5]Kung, HsiangFu(孔祥复)^[2,7]Tang, GuPing(汤谷平)*^[1,6]Lin, Marie C.*^[1,2]
语种：

英文
期刊：

BIOMATERIALS ISSN：0142-9612 2009 年 30 卷 29 期 (5793 - 5803) ; OCT
收录：
关键词：

Polyethylenimine beta-Cyclodextrin Folate Gene therapy Biodegradation In vivo test
摘要：

The success of gene therapy relies on a safe and effective gene delivery system. In this communication, we describe the use of folate grafted PEI600-CyD (H-1) as an effective polyplex-forming plasmid delivery agent with low toxicity. The structures of the polymer and polyplex were characterized, and the in vitro transfection efficiency, cytotoxicity, and in vivo transfection of H, were examined. We found that folate molecules were successfully grafted to PEI600-CyD. At NIP ratios between 5 and 30, the resulting H-1/DNA polyplexes had diameters less than 120 nm and zeta potentials less than 10 mV. In various tumor cell lines examined (U138, U87, B16, and Lovo), the in vitro transfection efficiency of H-1 was more than 50%, which could be improved by the presence of fetal bovine serum or albumin. The cytotoxicity of H, was significantly less than high molecular weight PEI-25 kDa. Importantly. in vivo optical imaging showed that the efficiency of H-1-mediated transfection (50 mu g luciferase plasmid (pLuc), N/P ratio = 20/1) was comparable to that of adenovirus-mediated luciferase transduction (1 x 10(9) pfu) in melanoma-bearing mice, and it did not induce any toxicity in the tumor tissue. These results clearly show that H, is a safe and effective polyp lex-forming agent for both in vitro and in vivo transfection of plasmid DNA and its application warrants further investigation. (C) 2009 Elsevier Ltd. All rights reserved.
推荐引用方式
GB/T 7714：

Yao Hong,Ng Samuel S.,Tucker Wesley O., et al. The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer [J].BIOMATERIALS,2009,30(29):5793-5803.
APA：

Yao Hong,Ng Samuel S.,Tucker Wesley O.,Tsang Yuk-Kai-Tiu,&Lin Marie C..(2009).The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer .BIOMATERIALS,30(29):5793-5803.
MLA：

Yao Hong, et al. "The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer" .BIOMATERIALS 30,29(2009):5793-5803.
入库时间：

2024/10/31 9:59:48
更新时间：

2024/10/31 9:59:48
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