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Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury 期刊论文

编号：

43589FB4994D6B25A88BDDE2C0337147
作者：

Wang, Chun#^[1,2]Jiang, Chen^[1];Yang, Yiran^[1];Xi, Cheng^[3];Yin, Yunxiang^[2];Wu, Haiying(吴海鹰)*^[4,5]Qian, Chuanyun(钱传云)*^[4,5]
语种：

英文
期刊：

IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES ISSN：2008-3866 2024 年 27 卷 3 期 (375 - 382) ; MAR
收录：
关键词：

Acute lung injury Exosomes Inflammation Interferon gamma Mesenchymal stem cells
摘要：

Objective(s): Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent stem cells with anti-inflammatory and immunomodulatory properties used in the treatment of acute lung injury (ALI). However, the treatment of ALI using exosomes derived from HUC-MSCs (HUC-MSCexos) primed with interferon-gamma (IFN-gamma-exos) has not been described. This study investigated the effects of IFN-gamma-exos on ALI. Materials and Methods: IFN-gamma primed and unprimed HUC-MSC-exos (IFN-gamma-exos and CONexos, respectively) were extracted, identified, and traced. A549 cells and mice subjected to lipopolysaccharide (LPS)-induced inflammation were treated with IFN-gamma-exos or CON-exos. Viability; interleukin (IL)-1 beta, IL-6, tumor necrosis factor (TNF)-alpha, and reactive oxygen species (ROS) levels; NF-KB p65, and NLRP3 expression and histology and lung injury scores were measured in cell, supernatant or lung tissue. Results: Indoleamine 2,3-dioxygenase (IDO) mRNA expression was elevated in HUC-MSCs primed with 5 ng/mL IFN-gamma (P<0.001), and IFN-gamma-exos and CON-exos were successfully extracted. LPSinduced inflammation resulted in decreased cell viability in A549 cells, and increased IL-1 beta, IL 6, TNF-alpha and ROS levels and NF-KB p65 and NLRP3 expression in A549 cells and mice(P<0.05 to P<0.001). Treatment with IFN-gamma-exos and CON-exos increased cell viability and decreased the concentrations of IL-1 beta, and ROS, expression of NF-KB p65 and NLRP3, and the lung injury score, and these effects were more obvious for IFN-gamma-exos(P<0.05 to P<0.001). Conclusion: IFN-gamma-exos reduced oxidative stress and inflammatory responses in LPS-induced A549 cells and mice. The result demonstrated the therapeutic potential of IFN-gamma-exos in LPS-induced ALI.
推荐引用方式
GB/T 7714：

Wang Chun,Jiang Chen,Yang Yiran, et al. Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury [J].IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES,2024,27(3):375-382.
APA：

Wang Chun,Jiang Chen,Yang Yiran,Xi Cheng,&Qian Chuanyun.(2024).Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury .IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES,27(3):375-382.
MLA：

Wang Chun, et al. "Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury" .IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 27,3(2024):375-382.
入库时间：

2024/3/31 1:19:53
更新时间：

2024/3/31 1:19:53
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