Background VB12 is involved in the production of bone marrow red blood cells, preventing pernicious anemia and brain nerve damage. Objective A large edible fungus Lactarius hatsudake (Lh) was used as a carbon source to produce fluorescent carbon dots (CDs) with high quantum yield and high photostability by a one-step hydrothermal method. Method The structure and optical properties of Lh-CDs were characterized by XRD, XPS, FTIR, and UV-Vis, and the stability of Lh-CDs was studied. The maximum excitation wavelength and emission wavelength of Lh-CDs were 324 and 408 nm, respectively, and the average fluorescence lifetime was 2.29 ns. Results The linear range is 0 similar to 20 mu M (R-2 = 0.9972) with a detection limit of 36.9 nM. At the same time, the mechanism of fluorescence quenching was internal filtration effect. Conclusions It showed that Lh-CDS had good selectivity, high sensitivity, and good anti-interference ability to VB12, which provided a new detection method for VB12.Highlights The fluorescence intensity of the LH-CDs could be effectively quenched by VB12.
[1]Kunming Med Univ, Dept Stomatol, Yanan Hosp, Kunming 650031, Yunnan, Peoples R China.
[2]Kunming Med Univ, Dept Minimally Invas Intervent, Affiliated Hosp 3, Kunming 650118, Yunnan, Peoples R China.
[3]Kunming Univ, Sch Chem & Chem Engn, Kunming 650214, Yunnan, Peoples R China.
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