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Human Dental Follicle Cell-Derived Small Extracellular Vesicles Attenuate Temporomandibular Joint Cartilage Damage through Inhibiting HIF-2α 期刊论文

编号：

8242546E18D9119CDBC5691C7BF01F1A
作者：

Mao, Enyu#^[1]Hu, Yu^[2];Xin, Yinzi^[2];Sun, Zheyi^[1];Zhang, Jun(张俊)^[1]Li, Song(李松)*^[3]
语种：

英文
期刊：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE ISSN：1932-6254 2023 年 2023 卷 ; SEP 25
收录：
摘要：

Mesenchymal stem cell (MSC)-based therapies for articular cartilage regeneration are effective mostly due to paracrine signals mediated by extracellular vesicles, especially small extracellular vesicles (sEV). However, it is unknown whether dental follicle cell-derived sEV (DFC-sEV) affect cartilage regeneration in temporomandibular joint osteoarthritis (TMJ-OA). In this study, the effects of DFC-sEV on IL-1 beta-induced mandibular condylar chondrocytes (MCCs) were determined using CCK8 assays, scratch assays, flow cytometry, and Western blot analysis of matrix synthesis and catabolic proteins. Furthermore, we used an abnormal occlusion-induced rat model and intra-articular injection of DFC-sEV, the pathological changes of which were observed by HE staining, safranin O staining, immunohistochemistry, and micro-CT analysis of subchondral bone loss. Gene set enrichment analysis (GSEA) was used to determine the related mechanism involved in the effect of DFC-sEV. Immunofluorescence analysis and Western blotting were used to evaluate the expression of HIF-1 alpha, HIF-2 alpha, MMP13, and VEGF in MCCs.)en, lentivirus-induced Epas1 overexpression and Western blot analysis of the downstream regulators of HIF-2 alpha were performed. We found that DFC-sEV promoted MCCs proliferation and migration and protected against cartilage matrix destruction induced by IL-1 beta. In addition, DFC-sEV prevented cartilage destruction in an abnormal occlusion rat model. Furthermore, we found that DFC-sEV reduced the expression of HIF-1 alpha and HIF-2 alpha in vitro and in vivo and decreased the downstream regulators of HIF-2 alpha, including MMP13 and VEGF. Our study indicated that DFC-sEV attenuated TMJ cartilage damage in vitro and in vivo, which might be involved in the regulation of HIF-2 alpha.
推荐引用方式
GB/T 7714：

Mao Enyu,Hu Yu,Xin Yinzi, et al. Human Dental Follicle Cell-Derived Small Extracellular Vesicles Attenuate Temporomandibular Joint Cartilage Damage through Inhibiting HIF-2α [J].JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2023,2023.
APA：

Mao Enyu,Hu Yu,Xin Yinzi,Sun Zheyi,&Li Song.(2023).Human Dental Follicle Cell-Derived Small Extracellular Vesicles Attenuate Temporomandibular Joint Cartilage Damage through Inhibiting HIF-2α .JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2023.
MLA：

Mao Enyu, et al. "Human Dental Follicle Cell-Derived Small Extracellular Vesicles Attenuate Temporomandibular Joint Cartilage Damage through Inhibiting HIF-2α" .JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 2023(2023).
入库时间：

2024/10/31 9:41:34
更新时间：

2024/10/31 9:41:34
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