The purpose of this study was to identify sesquiterpenoids from Alpinia oxyphylla Miq. fruits under the guidance of LC-MS, and to evaluate their neuroprotective effects on the H2O2-induced SH-SY5Y cells. A total of 35 ses-quiterpenoids, including 10 previously unreported ones, were isolated from A. oxyphylla fruits. The neuro-protective effect studies showed that compounds 2, 3, 12, 13, 20, 22, 25, 26, and 35 can improve the viability rates of the H2O2-induced SH-SY5Y cells whose viability rates were >= 80% and were higher than that of the positive control. Furthermore, thorough activity studies showed that compounds 3, 13, 22, and 35 can inhibit the production of ROS (reactive oxygen species), and that compounds 13, 22, and 35 can reduce both MDA (Malondialdehyde) and NO levels in the damaged cells in displaying a neuroprotective effect. This study confirmed that the fruits of A. oxyphylla contained abundant sesquiterpenoids with potential neuroprotective effect.
[1]Yunnan Univ Chinese Med, Sch Chinese Mat Med, Kunming 650500, Peoples R China.
[2]Yunnan Univ Chinese Med, Yunnan Key Lab Southern Med Utilizat, Kunming 650500, Peoples R China.
[3]Yunnan Agr Univ, Coll Trop Crops, Puer 665099, Peoples R China.
[4]Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China.
[5]Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China.
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